Incubation:
Article Title: Control of contextual memory through interneuronal α5-GABA A receptors.
Article Snippet: Slices were washed two times in PBS for 10 min and then incubated in primary antibody solution [0.2% Triton X-100, 10% normal goat serum (NGS; Thermo Fisher Scientific Cat# 50197Z), rabbit polyclonal anti-GABRA5 (1:200, Origene Cat# TA338505), and guinea pig polyclonal anti-GAD2 (1:200, Synaptic Systems Cat# 198 104) overnight at 4°C in a moist chamber with constant agitation (100 rpm)].
Article Title: Assemblies of Perisomatic GABAergic Neurons in the Developing Barrel Cortex
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GABA Sigma-Aldrich Cat#: A2052; RRID:AB_477652 Guinea pig polyclonal anti-GAD2 Synaptic Systems Cat#: 198104; RRID: AB_10557995 Guinea pig polyclonal anti-VGluT2 Merck Millipore Cat#: AB2251-I; RRID:AB_2665454 Mouse monoclonal anti-Syt2 Developmental Studies Hybridoma Bank Cat#: A2315626; RRID: AB_2315226 Bacterial and Virus Strains AAV1.syn.GCaMP6s.WPRE.SV40 UPenn viral core Cat#: 100843 Experimental Models: Organisms/Strains Swiss Webster (CFW) C.E Janvier SW-F/ SW-M Gad67.Cre a gift from H. Monyer Heidelberg University N/A RCL-GCaMP6s (Ai96) Jackson Laboratories 02 4106 SST.Cre a gift from N. Heintz, Rockefeller University N/A Lhx6.Cre Jackson Laboratories 026555 5HT3aR.Cre a gift from N. Heintz, Rockefeller University N/A GAD67-GFP-KI a gift from K. Obata Department of Morphological Brain Science, Kyoto N/A 5-HT3aR-GFP-KI a gift from G. Fishell Harvard University N/A Software and Algorithms CaImAn Pnevmatikakis et al., 2016 ; Giovannucci et al., 2018 https://github.com/flatironinstitute/CaImAn-MATLAB NoRMCorre Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/NoRMCorre Iterative K-means clustering Malvache et al., 2016 http://gitlab.com/cossartlab t-SNE Van Der Maaten and Hinton, 2008 https://lvdmaaten.github.io/tsne/#implementations Open in a separate window KEY RESOURCES TABLE In the developing barrel cortex, GABAergic neurons form functional assemblies Prospective parvalbumin interneurons segregate into spatially clustered assemblies Somatostatin interneurons display broadly correlated firing GABA assemblies are regulated by sensory inputs
Next-Generation Sequencing:
Article Title: Control of contextual memory through interneuronal α5-GABA A receptors.
Article Snippet: Slices were washed two times in PBS for 10 min and then incubated in primary antibody solution [0.2% Triton X-100, 10% normal goat serum (NGS; Thermo Fisher Scientific Cat# 50197Z), rabbit polyclonal anti-GABRA5 (1:200, Origene Cat# TA338505), and guinea pig polyclonal anti-GAD2 (1:200, Synaptic Systems Cat# 198 104) overnight at 4°C in a moist chamber with constant agitation (100 rpm)].
Article Title: Assemblies of Perisomatic GABAergic Neurons in the Developing Barrel Cortex
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GABA Sigma-Aldrich Cat#: A2052; RRID:AB_477652 Guinea pig polyclonal anti-GAD2 Synaptic Systems Cat#: 198104; RRID: AB_10557995 Guinea pig polyclonal anti-VGluT2 Merck Millipore Cat#: AB2251-I; RRID:AB_2665454 Mouse monoclonal anti-Syt2 Developmental Studies Hybridoma Bank Cat#: A2315626; RRID: AB_2315226 Bacterial and Virus Strains AAV1.syn.GCaMP6s.WPRE.SV40 UPenn viral core Cat#: 100843 Experimental Models: Organisms/Strains Swiss Webster (CFW) C.E Janvier SW-F/ SW-M Gad67.Cre a gift from H. Monyer Heidelberg University N/A RCL-GCaMP6s (Ai96) Jackson Laboratories 02 4106 SST.Cre a gift from N. Heintz, Rockefeller University N/A Lhx6.Cre Jackson Laboratories 026555 5HT3aR.Cre a gift from N. Heintz, Rockefeller University N/A GAD67-GFP-KI a gift from K. Obata Department of Morphological Brain Science, Kyoto N/A 5-HT3aR-GFP-KI a gift from G. Fishell Harvard University N/A Software and Algorithms CaImAn Pnevmatikakis et al., 2016 ; Giovannucci et al., 2018 https://github.com/flatironinstitute/CaImAn-MATLAB NoRMCorre Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/NoRMCorre Iterative K-means clustering Malvache et al., 2016 http://gitlab.com/cossartlab t-SNE Van Der Maaten and Hinton, 2008 https://lvdmaaten.github.io/tsne/#implementations Open in a separate window KEY RESOURCES TABLE In the developing barrel cortex, GABAergic neurons form functional assemblies Prospective parvalbumin interneurons segregate into spatially clustered assemblies Somatostatin interneurons display broadly correlated firing GABA assemblies are regulated by sensory inputs
Virus:
Article Title: Control of contextual memory through interneuronal α5-GABA A receptors.
Article Snippet: Slices were washed two times in PBS for 10 min and then incubated in primary antibody solution [0.2% Triton X-100, 10% normal goat serum (NGS; Thermo Fisher Scientific Cat# 50197Z), rabbit polyclonal anti-GABRA5 (1:200, Origene Cat# TA338505), and guinea pig polyclonal anti-GAD2 (1:200, Synaptic Systems Cat# 198 104) overnight at 4°C in a moist chamber with constant agitation (100 rpm)].
Article Title: Assemblies of Perisomatic GABAergic Neurons in the Developing Barrel Cortex
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GABA Sigma-Aldrich Cat#: A2052; RRID:AB_477652 Guinea pig polyclonal anti-GAD2 Synaptic Systems Cat#: 198104; RRID: AB_10557995 Guinea pig polyclonal anti-VGluT2 Merck Millipore Cat#: AB2251-I; RRID:AB_2665454 Mouse monoclonal anti-Syt2 Developmental Studies Hybridoma Bank Cat#: A2315626; RRID: AB_2315226 Bacterial and Virus Strains AAV1.syn.GCaMP6s.WPRE.SV40 UPenn viral core Cat#: 100843 Experimental Models: Organisms/Strains Swiss Webster (CFW) C.E Janvier SW-F/ SW-M Gad67.Cre a gift from H. Monyer Heidelberg University N/A RCL-GCaMP6s (Ai96) Jackson Laboratories 02 4106 SST.Cre a gift from N. Heintz, Rockefeller University N/A Lhx6.Cre Jackson Laboratories 026555 5HT3aR.Cre a gift from N. Heintz, Rockefeller University N/A GAD67-GFP-KI a gift from K. Obata Department of Morphological Brain Science, Kyoto N/A 5-HT3aR-GFP-KI a gift from G. Fishell Harvard University N/A Software and Algorithms CaImAn Pnevmatikakis et al., 2016 ; Giovannucci et al., 2018 https://github.com/flatironinstitute/CaImAn-MATLAB NoRMCorre Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/NoRMCorre Iterative K-means clustering Malvache et al., 2016 http://gitlab.com/cossartlab t-SNE Van Der Maaten and Hinton, 2008 https://lvdmaaten.github.io/tsne/#implementations Open in a separate window KEY RESOURCES TABLE In the developing barrel cortex, GABAergic neurons form functional assemblies Prospective parvalbumin interneurons segregate into spatially clustered assemblies Somatostatin interneurons display broadly correlated firing GABA assemblies are regulated by sensory inputs
Software:
Article Title: Control of contextual memory through interneuronal α5-GABA A receptors.
Article Snippet: Slices were washed two times in PBS for 10 min and then incubated in primary antibody solution [0.2% Triton X-100, 10% normal goat serum (NGS; Thermo Fisher Scientific Cat# 50197Z), rabbit polyclonal anti-GABRA5 (1:200, Origene Cat# TA338505), and guinea pig polyclonal anti-GAD2 (1:200, Synaptic Systems Cat# 198 104) overnight at 4°C in a moist chamber with constant agitation (100 rpm)].
Article Title: Assemblies of Perisomatic GABAergic Neurons in the Developing Barrel Cortex
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GABA Sigma-Aldrich Cat#: A2052; RRID:AB_477652 Guinea pig polyclonal anti-GAD2 Synaptic Systems Cat#: 198104; RRID: AB_10557995 Guinea pig polyclonal anti-VGluT2 Merck Millipore Cat#: AB2251-I; RRID:AB_2665454 Mouse monoclonal anti-Syt2 Developmental Studies Hybridoma Bank Cat#: A2315626; RRID: AB_2315226 Bacterial and Virus Strains AAV1.syn.GCaMP6s.WPRE.SV40 UPenn viral core Cat#: 100843 Experimental Models: Organisms/Strains Swiss Webster (CFW) C.E Janvier SW-F/ SW-M Gad67.Cre a gift from H. Monyer Heidelberg University N/A RCL-GCaMP6s (Ai96) Jackson Laboratories 02 4106 SST.Cre a gift from N. Heintz, Rockefeller University N/A Lhx6.Cre Jackson Laboratories 026555 5HT3aR.Cre a gift from N. Heintz, Rockefeller University N/A GAD67-GFP-KI a gift from K. Obata Department of Morphological Brain Science, Kyoto N/A 5-HT3aR-GFP-KI a gift from G. Fishell Harvard University N/A Software and Algorithms CaImAn Pnevmatikakis et al., 2016 ; Giovannucci et al., 2018 https://github.com/flatironinstitute/CaImAn-MATLAB NoRMCorre Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/NoRMCorre Iterative K-means clustering Malvache et al., 2016 http://gitlab.com/cossartlab t-SNE Van Der Maaten and Hinton, 2008 https://lvdmaaten.github.io/tsne/#implementations Open in a separate window KEY RESOURCES TABLE In the developing barrel cortex, GABAergic neurons form functional assemblies Prospective parvalbumin interneurons segregate into spatially clustered assemblies Somatostatin interneurons display broadly correlated firing GABA assemblies are regulated by sensory inputs
Functional Assay:
Article Title: Control of contextual memory through interneuronal α5-GABA A receptors.
Article Snippet: Slices were washed two times in PBS for 10 min and then incubated in primary antibody solution [0.2% Triton X-100, 10% normal goat serum (NGS; Thermo Fisher Scientific Cat# 50197Z), rabbit polyclonal anti-GABRA5 (1:200, Origene Cat# TA338505), and guinea pig polyclonal anti-GAD2 (1:200, Synaptic Systems Cat# 198 104) overnight at 4°C in a moist chamber with constant agitation (100 rpm)].
Article Title: Assemblies of Perisomatic GABAergic Neurons in the Developing Barrel Cortex
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GABA Sigma-Aldrich Cat#: A2052; RRID:AB_477652 Guinea pig polyclonal anti-GAD2 Synaptic Systems Cat#: 198104; RRID: AB_10557995 Guinea pig polyclonal anti-VGluT2 Merck Millipore Cat#: AB2251-I; RRID:AB_2665454 Mouse monoclonal anti-Syt2 Developmental Studies Hybridoma Bank Cat#: A2315626; RRID: AB_2315226 Bacterial and Virus Strains AAV1.syn.GCaMP6s.WPRE.SV40 UPenn viral core Cat#: 100843 Experimental Models: Organisms/Strains Swiss Webster (CFW) C.E Janvier SW-F/ SW-M Gad67.Cre a gift from H. Monyer Heidelberg University N/A RCL-GCaMP6s (Ai96) Jackson Laboratories 02 4106 SST.Cre a gift from N. Heintz, Rockefeller University N/A Lhx6.Cre Jackson Laboratories 026555 5HT3aR.Cre a gift from N. Heintz, Rockefeller University N/A GAD67-GFP-KI a gift from K. Obata Department of Morphological Brain Science, Kyoto N/A 5-HT3aR-GFP-KI a gift from G. Fishell Harvard University N/A Software and Algorithms CaImAn Pnevmatikakis et al., 2016 ; Giovannucci et al., 2018 https://github.com/flatironinstitute/CaImAn-MATLAB NoRMCorre Pnevmatikakis and Giovannucci, 2017 https://github.com/flatironinstitute/NoRMCorre Iterative K-means clustering Malvache et al., 2016 http://gitlab.com/cossartlab t-SNE Van Der Maaten and Hinton, 2008 https://lvdmaaten.github.io/tsne/#implementations Open in a separate window KEY RESOURCES TABLE In the developing barrel cortex, GABAergic neurons form functional assemblies Prospective parvalbumin interneurons segregate into spatially clustered assemblies Somatostatin interneurons display broadly correlated firing GABA assemblies are regulated by sensory inputs
|
Synaptic Systems is a verified supplier 
Synaptic Systems manufactures this product 